Weather: Sunny Stadium: Palmerston Park Pitch: Good Round: 19
Home Manager: Gary Andrew Naysmith Away Manager: Ian McCall
| Scotland Championship | 2016/10/22 14:00 | Ayr v Queen of South | L | 1-0 |
| Scotland Championship | 2016/08/13 14:00 | Queen of South v Ayr | W | 4-1 |
| Scotland Championship | 2016/12/24 13:00 | Morton v Queen of South | L | 1-0 |
| Scotland Championship | 2016/12/17 15:00 | Queen of South v Dunfermline | D | 2-2 |
| Scotland Championship | 2016/12/10 15:00 | Falkirk v Queen of South | D | 2-2 |
| Scotland Championship | 2016/12/06 19:45 | Queen of South v St Mirren | L | 2-3 |
| Scotland Championship | 2016/12/03 15:00 | Queen of South v Dumbarton | L | 1-2 |
| Scotland FA Cup | 2016/11/29 19:45 | Albion v Queen of South | L | 2-1 |
| Scotland Championship | 2016/11/19 15:00 | Hibernian v Queen of South | L | 4-0 |
| Scotland League Challenge Cup | 2016/11/12 15:00 | Queen of South v Alloa | W | 2-0 |
| Scotland Championship | 2016/12/24 12:30 | Ayr v Dumbarton | D | 4-4 |
| Scotland Championship | 2016/12/17 15:00 | St Mirren v Ayr | D | 1-1 |
| Scotland Championship | 2016/12/10 15:00 | Ayr v Dundee Utd | L | 0-1 |
| Scotland Championship | 2016/12/03 15:00 | Dunfermline v Ayr | D | 1-1 |
| Scotland FA Cup | 2016/11/29 19:45 | Brechin v Ayr | W | 0-1 |
| Scotland Championship | 2016/11/22 19:45 | Raith v Ayr | D | 1-1 |
| Scotland Championship | 2016/11/19 15:00 | Ayr v Falkirk | L | 0-1 |
| Scotland League Challenge Cup | 2016/11/13 12:30 | St Mirren v Ayr | L | 2-1 |